Genetics and Bio lab techniques
Posted on February 2, 2019
Tags: biochem
Sequencing :: gene -> expression
Expression
Microarray :: (Vivo x) => Known-genes x -> expression-levels x
RNA-Seq :: (Vivo x) => RNA x -> expression-levels x
Known-genes ⊆ RNA
--(Vivo x) => .. is a type class RNA-Sequencing is expensive compared to microarrays
RNA-Seq
- RNA-Seq
- Make cDNA library from RNA
- Chop up the pieces
1 Crossing
type Chromosome x = x :: (Chromosome x) | Nil
-- ex.
type Genome a b = (Chromosome a, Chromosome b)
crossing :: Genome a b -> Genome a b -> Genome a b
crossing [Cha_Gal4, Cha_L13] []- Phenotypical markers vs Genotypical markers
2 FLP-FRT recombination
Insertion = plasmid[..Frt,i,Frt..] + [a,Frt,b] -> [a,Frt,i,Frt,b]
Deletion = [a,Frt,i,Frt,b] -> [a,Frt,b]
Inversion = [a,Frt,Seq,Frt,b] -> [a,Frt,rev(Seq),Frt,b]
Transitivity = [a,Frt,x] -> [y,Frt,b] -> [a,Frt,b] 
